Mapping Protein–Protein Interactions Using Yeast Two-Hybrid Assays
نویسندگان
چکیده
منابع مشابه
Mapping protein-protein interactions using yeast two-hybrid assays.
Yeast two-hybrid (Y2H) screens are an efficient system for mapping protein-protein interactions and whole interactomes. The screens can be performed using random libraries or collections of defined open reading frames (ORFs) called ORFeomes. This protocol describes both library and array-based Y2H screening, with an emphasis on array-based assays. Array-based Y2H is commonly used to test a numb...
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The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2015
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot086157